total extracts drg Search Results


human drg  (TaKaRa)
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minibest universal rna extraction kit  (TaKaRa)
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TaKaRa minibest universal rna extraction kit
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rneasy kit  (Qiagen)
90
Qiagen rneasy kit
Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy mini kit  (Qiagen)
96
Qiagen rneasy mini kit
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total extracts drg  (Proteintech)
93
Proteintech total extracts drg
List of primary antibodies
Total Extracts Drg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total extracts drg  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc total extracts drg
List of primary antibodies
Total Extracts Drg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total rna extraction kit  (Promega)
90
Promega total rna extraction kit
List of primary antibodies
Total Rna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superscripttm vilotm cdna synthesis kit  (Thermo Fisher)
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Thermo Fisher superscripttm vilotm cdna synthesis kit
List of primary antibodies
Superscripttm Vilotm Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy micro kit  (Qiagen)
97
Qiagen rneasy micro kit
List of primary antibodies
Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy  (Qiagen)
90
Qiagen rneasy
List of primary antibodies
Rneasy, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total extracts drg  (Abcam)
98
Abcam total extracts drg
List of primary antibodies
Total Extracts Drg, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


List of primary antibodies

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

Techniques: Isolation

Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation

List of primary antibodies

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

Techniques: Isolation

Effect of short-term diabetes on proteins involved in mitochondrial fission/fusion: MFN2, Drp1 and OPA1. a Immunoblot of DRP1 phosphorylation from DRG total extracts of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. The bar graph represents densitometric analysis and statistical evaluation of DRP1 phosphorylation over total DRP1 (DRP1P/DRP1 tot); representative western blot of phosphorylated and total DRP1 is presented above the graph. b Immunoblot of MFN2 from DRG total extracts of Ctrl and STZ M and F rats. c Immunoblot of OPA1 from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05) followed by Bonferroni post hoc test (significance: * p < 0.05)

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on proteins involved in mitochondrial fission/fusion: MFN2, Drp1 and OPA1. a Immunoblot of DRP1 phosphorylation from DRG total extracts of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. The bar graph represents densitometric analysis and statistical evaluation of DRP1 phosphorylation over total DRP1 (DRP1P/DRP1 tot); representative western blot of phosphorylated and total DRP1 is presented above the graph. b Immunoblot of MFN2 from DRG total extracts of Ctrl and STZ M and F rats. c Immunoblot of OPA1 from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05) followed by Bonferroni post hoc test (significance: * p < 0.05)

Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

Techniques: Western Blot

List of primary antibodies

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: Mitoprofile total OXPHOS , Total Extracts DRG , 1:1.000 , Abcam (ab10413).

Techniques: Isolation

Effect of short-term diabetes on PGC-1α gene expression, respiratory chain complex IV expression and mitochondrial DNA. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of PGC1-α. b Immunoblot of mitochondrial OXPHOS Complex IV from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graph, with the respective loading control (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. c Total DNA was isolated from non-diabetic Ctrl and STZ rats and run for quantitative real-time PCR to obtain a relative ratio of mt-CoxII (a gene coded on the mitochondrial genome) over 36B4 (a gene coded on nuclear genome), as indicator for relative mt-DNA copy number. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sexby diabetes were analyzed using two-way ANOVA (significance: ## p < 0.01; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05; **p < 0.01)

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on PGC-1α gene expression, respiratory chain complex IV expression and mitochondrial DNA. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of PGC1-α. b Immunoblot of mitochondrial OXPHOS Complex IV from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graph, with the respective loading control (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. c Total DNA was isolated from non-diabetic Ctrl and STZ rats and run for quantitative real-time PCR to obtain a relative ratio of mt-CoxII (a gene coded on the mitochondrial genome) over 36B4 (a gene coded on nuclear genome), as indicator for relative mt-DNA copy number. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sexby diabetes were analyzed using two-way ANOVA (significance: ## p < 0.01; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05; **p < 0.01)

Article Snippet: Mitoprofile total OXPHOS , Total Extracts DRG , 1:1.000 , Abcam (ab10413).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation, Real-time Polymerase Chain Reaction